Modification of sample processing for the Limulus amebocyte lysate assay enhances detection of inflammogenic endotoxin in intact bacteria and organic dust
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چکیده
منابع مشابه
Single-step, chromogenic Limulus amebocyte lysate assay for endotoxin.
A new reagent for the chromogenic Limulus amebocyte lysate (LAL) assay is described. LAL was formulated for optimal performance in either an endpoint procedure or a kinetic procedure with the chromogenic substrate, buffer, and LAL components colyophilized as a single reagent. The kinetic chromogenic method required an incubating microplate reader coupled to a computer for collection and analysi...
متن کاملMeasuring of free endotoxin in alum-precipitated vaccine of haemorrhagic septicaemia by limulus amebocyte lysate test
Haemorrhagic septicaemia (HS) vaccine which is prepared in Razi Institute is used in endemic areas ofIran. Aluminum-hydroxide gel was used as adjuvant for preparing this vaccine. Post-vaccinal shock reactionswere the main complaint after use of this vaccine. In a previous study, we could improve the vaccine byalum-precipitation Pasteurella multocida cells and removing the liquid phase. In this ...
متن کاملAirborne endotoxin from indoor and outdoor environments: effect of sample dilution on the kinetic Limulus amebocyte lysate (LAL) assay.
Airborne endotoxins in occupational environments are a potential respiratory hazard to individuals. In this study, airborne endotoxins were collected using open-face and button aerosol samplers from inside animal housing units and downwind from agricultural production sites and a wastewater treatment plant. Filter extracts were then diluted to examine the effect of interfering substances on the...
متن کاملEndotoxins and Their Detection with the Limulus Amebocyte Lysate Test
In contrast to the detailed descriptions of techniques provided by Larsson, a chapter on the measurement and analysis of acetylcholine written by Israel Hanin takes the form of a brief but comprehensive survey of all of the methods for the quantitation of acetylcholine introduced between 1933 and 1981. Each method presented is referenced and briefly critiqued. This is followed by an article by ...
متن کاملModified micromethod of the Limulus amoebocyte lysate assay for endotoxin.
A Limulus amoebocyte lysate microtechnique performed in petrolatum wells on a microscope slide is described. Injection of a dye solution in ethanol directly into the wells leads to an unambiguous interpretation of the results. Twelve samples can be tested on a single slide, and compact storing of the samples is possible.
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ژورنال
عنوان ژورنال: Innate Immunity
سال: 2017
ISSN: 1753-4259,1753-4267
DOI: 10.1177/1753425917694084